Therapeutic Effects of Topical Application of Ozonated Olive Oil on Acute Cutaneous Wound Healing

   In 2009 a group of scientists (Hee Su Kim, Sun Up Noh, Ye Won Han, Kyoung Moon Kim, Hoon Kang, Hyung Ok Kim and Young Min Park) from Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea, undertook a study in which they researched  the therapeutic effects of topical ozonated olive oil on acute cutaneous wound healing in a guinea pig model and also to elucidate its therapeutic mechanism. After creating full-thickness skin wounds on the backs of guinea pigs by using a 6 mm punch biopsy, they examined the wound healing effect of topically applied ozonated olive oil (ozone group), as compared to the pure olive oil (oil group) and non-treatment (control group). The ozone group of guinea pig had a significantly smaller wound size and a residual wound area than the oil group, on days 5 (P<0.05) and 7 (P<0.01 and P<0.05) after wound surgery, respectively. Both hematoxylin-eosin staining and Masson-trichrome staining revealed an increased intensity of collagen fibers and a greater number of fibroblasts in the ozone group than that in the oil group on day 7. Immunohistochemical staining demonstrated upregulation of platelet derived growth factor (PDGF), transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF) expressions, but not fibroblast growth factor expression in the ozone group on day 7, as compared with the oil group. In conclusion, these results demonstrate that topical application of ozonated olive oil can accelerate acute cutaneous wound repair in a guinea pig in association with the increased expression of PDGF, TGF-β, and VEGF.

  

INTRODUCTION

   The closure of cutaneous wounds involves complex tissue movements such as hemorrhage, inflammation, re-epithelization, granulation tissue formation, and the late remodeling phase of repair . These events involve coordination of dozens of types of cells and matrix proteins, which are all important to control stages of the repair process. Previous studies have demonstrated that endogenous growth factors, such as fibroblast growth factors (FGF), platelet derived growth factors (PDGF), transforming growth factor-β (TGF-β) and vascular endothelial growth factors (VEGF) are the important regulatory polypeptides for coordinating the healing process. They are released from macrophages, fibroblasts, and keratinocytes at the site of injury and they participate in the regulation of re-epithelization, granulation tissue formation, collagen synthesis and neovascularization.

   Ozonated oil has been widely recognized as one of the best bactericidal, antiviral and antifungal agents and it has been used empirically as a clinical therapeutic agent for chronic wounds, such as trophic ulcers, ischemic ulcers and diabetic wounds. The beneficial effects of ozonated oil on wound healing might be assumed to be due to decreased bacterial infection, ameliorated impaired dermal wound healing or increased oxygen tension by ozonated oil exposure in the wound area.

   It was reported that O3 exposure is associated with activation of transcription factor NF-κB; this is important to regulate inflammatory responses and eventually the entire process of wound healing. It was shown that huge amounts of PDGF and TGF-β1 were released from platelets in the heparinized plasma of a limb ischemia patient after ozonation. It has been revealed that there were substantial increases of steady-state mRNA levels of TGF-β1 in the fibroblasts that were co-cultured with bronchoepithelial cells after O3 exposure. A recent study has shown that hydrogen peroxide (H2O2) potently induced the VEGF expression in human keratinocytes which can stimulate wound healing. From these previous studies of O3, we hypothesized that O3 might enhance acute cutaneous wound healing, and this could be associated with growth factors such as FGF, PDGF, TGF-β and VEGF.

    Nowadays, O3 is profitably and practically employed as ozonated olive oil; this contains the O3 molecule stabilized as an ozonide between the double bonds of a monounsaturated fatty acid such as oleic acid, which is ideal for the topical use of O3 to treat chronically infected cutaneous and mucosal areas of the body. Ozonated materials referred to as ozonides are formed by the reaction of olefins with ozone. Any olefin can be treated with gaseous ozone to form an ozonide. The ozonide compositions have the capacity to deliver nascent oxygen deep within the lesion without causing primary skin irritation.

     Ozonated oil has been used topically for the treatment of chronic wounds, but there have been few studies concerning with the therapeutic effects of ozonated olive oil on acute cutaneous wound healing in animal models. The present study was designed to evaluate the therapeutic effect of topical ozonated olive oil on acute cutaneous wound healing in a guinea pig model, and to elucidate its therapeutic mechanisms that are associated with such growth factors as FGF, PDGF, TGF-β, and VEGF.

    For the experiment, the researchers team used sixteen female guinea pigs (400-450 g), aged 8-9 weeks, were placed in a room that was under a 12-hr light and 12-hr dark cycle. The animals were placed individually in separate cages with ad libitum access to food and water. The animal care, handling, and experimental procedures were carried out in accordance with a protocol approved by the Animal Care and Use Committee of the Catholic University of Korea.

   After 7 days of acclimation, the guinea pigs were anesthesized with ketamine and their backs were shaved and then sterilized with normal saline. The skin was then pinched and folded, and a sterile biopsy punch (6 mm in diameter, Stiefel Co., Offenbach, Germany) was used to make a full-thickness hole in the skin. Two wounds were created on both sides of the back for a total 4 circular wounds per animal.

Application of topical ozonated olive oil

   Two drops (about 0.1 mL) of ozonated olive oil were applied everyday to two sites of the four wounds (Ozone group). Olive oil as a pure base was applied to a third wound (Oil group). As a control group, nothing was applied on the fourth wound. The wounds were then dressed with Opsite® to cover them without dryness. An elastic bandage was wrapped around the area to prevent further injury.  

   On days 3 (n=4), 7 (n=4), and 11 (n=8) after wounding, guinea pigs were euthanized using ketamine and the four wounded tissues from each guinea pig were excised. Then the tissues were cut into halves; one half was placed in formalin (10% formaldehyde in phosphate-buffered saline) for hematoxylin-eosin and Masson-trichrome staining, and the second half was quickly kept as a frozen state (-80℃) for immunohistochemistry.

Histology

   The specimens for histological examination were collected from each group by the full-thickness excision. Intensity of the collagen fibers and the fibroblast proliferation were examined under microscope in hematoxylin-eosin and Masson-trichrome staining. The staining intensity of the collagen fibers was graded under ×200 magnification as follows: -, completely negative staining intensity; ±, lower staining intensity; +, moderate staining intensity; ++, slightly higher staining intensity; +++, considerably higher staining intensity. The number of fibroblasts was counted in the 5 randomized fields per specimen under ×400 magnification. Three dermatologists, who were "blinded" to which groups the specimens were in, independently analyzed all the specimens and then the mean numbers and standard deviations of the fibroblasts in all groups of wound were calculated, respectively.

Result

The ozonated ozonated oil significantly enhances the acute cutaneous wound healing

As shown in Fig. 1, there was an enhanced wound closure in the ozone group of wounds, the left two of the four wounds, as compared to the oil group as well as the control group. On day 11, all of the wounds completely re-epithelized irrespective of treatments. The ozone group showed a significantly smaller wound size than the oil group on days 5 (P<0.05) and 7 (P<0.01). The ozone group showed 58%, 46.3%, and 16.4% in residual wound area on days 3, 5 and 7, respectively. On the other hand, the oil group revealed 61.8%, 53.7% and 25.6% in residual wound area, respectively (Table 1). Thus, there was a significant difference in the residual wound area between the ozone group and the oil group on days 5 (P<0.05) and 7 (P<0.01). On repeated-measures of ANOVA, the ozone group showed a significantly decreased residual wound area as compared to the oil group, as well as the control group (P<0.05).

fig.1 The effects of ozonated olive oil on clinical wound closure. The photomicrographs demonstrate the enhanced wound closure, in the ozone group, on the left two wounds (a and b) on the back of the guinea pig, as compared to the oil group (c) as well as the control group (d). (Bar=5 mm).

Tabel 1 Comparison of the average wound size and residual wound area on post-operation days 0, 3, 5, 7, and 11

The ozonated ozonated oil promotes collagen synthesis and fibroblast proliferation

  On the hematoxylin-eosin staining, epithelization, infiltration of inflammatory cells and vascular proliferations were commonly seen on all of the wounds; but there were proliferation of fibroblasts and collagen fibers noted in the ozone group as well (data not shown). We performed the Massontrichrome staining in order to determine whether ozonated OLO's ability to accelerate wound closure was associated with collagen synthesis and fibroblast proliferation at the wound bed and at the edge of the injury site. The staining intensity of collagen fibers and the number of fibroblasts were evaluated on days 3 and 7. On day 3, the ozone group did not show a significant difference in the staining intensity of collagen fibers and the number of fibroblasts as compared to the oil group. In contrast, on day 7, the ozone group revealed an increased staining intensity of collagen fibers at the wound bed and at the edge of the entire dermis in comparison to the oil and control groups (Fig.2). On day 7, the numbers of fibroblasts in the ozone group were 62.3 and 35.6 at the wound bed and edge, respectively; but, the numbers of fibroblasts in the oil group were 48.5 and 22.7, respectively (Table 2). Thus, there was a significant difference in the staining intensity of collagen fibers and the number of fibroblasts between the ozone group and the oil group on day 7 (P<0.05), but not on day 3.

Fig. 2.

Masson-trichrome staining of the wound bed and the edge of the injury site on days 3 and 7. The ozone group revealed the increased staining intensity of collagen fibers and the number of fibroblasts at the wound bed and edge, in comparison to the oil group and the control group on day 7, but not on day 3 (original magnification ×400, Bar=50 µm).
Table 2.
Comparison of staining intensity of collagen fibers and the number of fibroblasts by the Masson-trichrome staining on days 3 and 7

 

The ozone group revealed increased expressions of PDGF, TGF-β, and VEGF, but not FGF

  In order to determine which growth factors play an important role for the accelerating wound closure associated with the proliferation of fibroblasts and collagen fibers by the ozonated OLO, we evaluated the immunohistochemical staining intensity of FGF, PDGF, TGF-β, and VEGF on day 7 (Fig.3). FGF expressions were identified in dermal fibroblasts and collagen fibers, but this was barely detected in the epidermis of the control group. There were little differences in the FGF expression between the ozone group and the oil group (Table 3). PDGF was expressed in dermal inflammatory cells, fibroblasts, epidermal cells and keratinocytes of hair follicles of the control group. The ozone group revealed a relatively higher PDGF expression, as compared to the oil group. There was a relatively distinct difference in the dermis between the former and the latter. TGF-β expressions were detected in the dermal fibroblasts, epidermal cells and keratinocytes of the hair follicles of the control group. Likewise, the ozone group showed a relatively increased expression of TGF-β, as compared to the oil group. VEGF expression was identified in dermal fibroblasts, endothelial cells and collagen fibers, but it was barely detected in the epidermis of the control group. The ozone group revealed a relatively increased VEGF expression in both the dermis and the epidermis, as compared to the oil group. These findings demonstrated that the ozone group revealed relatively higher expressions of PDGF, TGF-β, and VEGF, but not FGF than the oil group on day 7.

Fig. 3 Immunohistochemical staining for FGF, PDGF, TGF-β, and VEGF on day 7. The ozone group revealed the relatively increased expressions of PDGF (BFJ), TGF-β (CGK), and VEGF (DHL), but not FGF (AEI) as compared to the oil group on day 7 (×100, original magnification, inlet; ×400, Bar=500 µm).

Table 3.

Comparison of the expression of growth factors on day 7

Discussion

   Based on the results from the wound closure between the ozone group and the oil group on days 0, 3, 5, 7, and 11, the ozone group showed a significantly smaller wound size and residual wound area than the oil group in the guinea pig model on days 5 and 7. Thus, these results demonstrated that O3 could enhance acute cutaneous wound healing. Especially, on day 7, both the wound size and residual wound area of the ozone group were much more significantly decreased. This implies that topical exposure of O3 may affect granulation tissue formation of the wound healing process rather than affecting immediate formation of blood clot and recruitment of inflammatory cells during the inflammation phase.

  On Masson-trichrome staining on day 3, there was not much difference in the staining intensity of collagen fibers and fibroblast proliferation between the ozone group and the oil group. However, on day 7, the ozone group revealed about one and half times increased staining intensity of collagen fibers and significantly increased fibroblasts at the wound edge as well as at the wound bed, compared to the oil group. These findings indicated that O3 may act on acute wound healing directly or indirectly via collagen synthesis and fibroblast proliferation during granulation tissue formation and the early tissue remodeling phase of wound healing.

  Fibroblasts have been known to play important roles in reepithelization, collagen fiber synthesis, extracellular matrix regeneration, remodeling of wounds, and for the release of such endogenous growth factors as FGF, PDGF, TGF-β, and VEGF. In this study, increased expressions of PDGF and TGF-β were seen in the ozone group on day 7, which is correlated with increased staining intensity of collagen fibers and fibroblast proliferation on the same day. There were also increased expressions of PDGF and TGF-β in the epidermal keratinocytes and the hair follicular cells adjacent to the injury. These findings suggest that O3 might induce expressions of PDGF and TGF-β from epidermal keratinocyte as well as from the dermal fibroblast at the injury site. In the present study, there was a relatively increased expression of VEGF in the epidermal keratinocytes of the ozone group on day 7; this was consistent with the previous study showing that VEGF was gradually increased from the 1st day to the 7th day of normal wound healing process. On hematoxylin-eosin staining, the relatively increased vascularity in the ozone group might be due to the increased expression of VEGF by ozonation. These findings may be associated with the generation of H2O2 through ozonation, which can directly induce a VEGF expression and/or indirectly induce it by the induction of heme oxygenase-1. Because VEGF is main cytokine of vascularization in the late phase of wound healing, further study will be needed to clarify the effect of O3 on the neovascularization of cutaneous wound healing. In contrast to the increased expressions of PDGF, TGF-β, and VEGF in the ozone group, there was little difference in the FGF expressions between the ozone group and the oil group on day 7. One of the possible explanations for this result is that FGF had already been up-regulated within 24 hr after wounding.

In conclusion, these results demonstrate that application of ozonated olive oil , the topical form of O3, can accelerate acute cutaneous wound repair in the guinea pig model by promoting collagen synthesis and fibroblast proliferation at the injury site and by increasing the expression of growth factors such as PDGF, TGF-β, and VEGF. Taken together, we can infer that topical O3 may be regarded as an alternative therapeutic modality to enhance cutaneous wound healing.

Sources:

Therapeutic Effects of Topical Application of Ozone on Acute Cutaneous Wound Healing

Hee Su Kim, Sun Up Noh, Ye Won Han, Kyoung Moon Kim, Hoon Kang, Hyung Ok Kim and Young Min Park

Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

 

 


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